Composite

Part:BBa_K1604021:Design

Designed by: Veronica Pinamonti   Group: iGEM15_UNITN-Trento   (2015-08-18)


J23100 + β-carotene 15,15’-dioxygenase (blh gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 245
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was subcloned from pUC57 into pSB1C3


Source

The sequence of uncultered cluster bacteria SAR86 was synthetized and codon optimized by GeneScript

References